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It's a good way to test a syringe too. So you can introduce them to your lc or your agar.
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On top of all this, they’re very easy to make at home and only require a few ingredients.
How to make spore syringe from agar. Wrap your petri dish with parafilm. Shake up the spore syringe till the spores start to break apart. Keep agar plate level until drop is absorbed.
Click a timestamp to jump to that section of video. Spore syringes make it easy to make grain spawn or start new agar colonies in a snap. There are a couple of techniques you can use to make the spore syringe.
Making a spore syringe materials. Take a syringe of sterile water flame sterilize the needle and inject 2cc of water into the petri dish on top of the growth. The most common method is to transfer 10 ml of the sterile water to a shot glass, or a small glass cup, then while gently holding the spore print, use a scalpel to scrape the spores into the water.
As they cool, you can place them in the box. Keep agar plate level until drop is absorbed. Scrape the mycelia growth loose into the drop of water then suck it back up into the syringe.
The only way to use a syringe with bacterial tam that shows on agar or lc is grain spawn, or cakes. You don't need much to start. Shake well, flame needle, squirt a bit out to cool needle, place one drop on center of agar.
A lot of times, if they are not your spore syringes that you made from your own prints, you just introduce tams to the agar. Flame sterilize the needle again and inject the strands into an lc. Flame the needle for 30 seconds to remove any contamination.
It is better to go print to agar but this works fine. After 30 seconds wipe off the needle with an alcohol wipe. Drop a few squirts of spores onto the agar.
If the print you used to make the spore syringe is from a clean indoor grow, you could expect a high. Spore syringes make it easy to make grain spawn or start new agar colonies in a snap. For example, stir up the mycelium a bit from the agar plate, add some sterile water and suck that into a syringe.
These syringes keep spores hydrated and clean making them easy to transfer to a sterile environment.
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